Laboratoire de Chimie Bactérienne UMR 7283



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IMM ERC ANR FRM CENTURI Bettencourt Arc Arc Arc PACA mucoviscidose Amidex CNRS Formation

Accueil > Research > PY > Theme

Fe-S clusters biogenesis and homeostasis

Our research, using E. coli as a model organism, has focused on deciphering how cells ensure the essential biological process that is Fe-S proteins biogenesis, especially under conditions that are detrimental for Fe-S clusters. Our project takes advantage of our well-established expertise in genetics and biochemical approaches to further develop an “integrative” view of Fe-S cluster-containing protein biogenesis. In particular we aim at deciphering Fe-S cluster assembly, Fe-S cluster insertion/delivery routes and Fe-S cluster regulatory genetic control, to establish an integrated model for Fe-S cluster protein biogenesis. Also, using imaging techniques and including another model organism we expect to open new perspectives on Fe-S homeostasis and biogenesis. 

Fe-S biogenesis factors of E. coli.
Fe-S protein biogenesis starts with de novo Fe-S cluster assembly on a scaffold protein, by a set of components that includes the sulfur donor, namely a cysteine desulfurase generating a persulfide on its active-site cysteine. Once assembled on the scaffold, Fe-S clusters are delivered to apo-targets thanks to Fe-S carriers 

Genetic regulation of the E. coli isc and suf operons 

The questions we are interested in : 
i) The molecular mechanism of Fe-S cluster biogenesis.
 Functioning of the Fe-S assembly complex ?
Are there any additional biogenesis factors and if so, what are their roles ?
How to make Fe-S routes compatible with “foreign” Fe-S proteins ? 
ii) The genetic regulation to establish Fe-S homeostasis.
Which types of genetic regulation are involved ? 
iii) Fe-S biogenesis and homeostasis in a other model bacterium than E. coli.