Laboratoire de Chimie Bactérienne UMR 7283

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15 juin 2017: 1 événement


  • Séminaire LCB

    Jeudi 15 juin 2017 14:30-16:00 - Dr. Matteo Brilli (invité par Emanuele Biondi) - Université de Padoue

    Multi-omics dissection of the Plasmopara viticola - Vitis vinifera pathosystem : from NGS data to mathematical models.

    Résumé : Wine production is worth more than 25 billion euros and it is challenged by a few major pathogens such as downy mildew, caused by the Oomycete Plasmopara viticola, and powdery mildew, caused by the fungus Erisyphe necator. When the weather is optimal for the pathogen downy mildew can destroy more than 75% of the crop such that abundant fungicide treatments are required : in wet years, 90% of the fungicide sprays are targeted to downy and powdery mildews, a market of over €1.5 billions/year in Europe only. However, fungicides have long-term effects on the environment and likely on animals, asking for alternative control strategies, but their development is hampered by the poor knowledge of the molecular details of the P. viticola infection. To fill this gap, we present a draft genome assembly of P. viticola and we analyze its gene content in a comparative genomics framework to understand the basis for biotrophy and to identify genes and biological processes involved in the infection process. In addition, RNA-Seq was performed during the infection to identify plant and Plasmopara genes that are (differentially) expressed. Small RNA-Seq was also performed at several time points during infection to produce the first libraries of sRNAs from P. viticola and infected grapevine and it was paralleled by degradome sequencing in infected and non-infected conditions, to support the prediction of regulatory interactions mediated by sRNA.
    The main finding of our work is the existence of extensive exchanges of small-RNAs among the pathogen and the host that possibly result in important regulations ; while requiring additional experimental confirmation, our findings point towards possible control strategies based on artificial sRNA regulators.
    Results in this work are also discussed in the light of integrative strategies in prokaryotic research to fill the gap from sequences (NGS) to predictive models of metabolism and gene regulation.

    Lieu : Jacques Senez

    En savoir plus : Séminaire LCB