Adaptation au stress chez les entérobactéries
Keywords : Escherichia coli, Salmonella Typhimurium, Fe/S biogenesis, Stress response, sncRNA, Methionine Sulfoxide reductase.
Bacterial stress adaptation is the underlying theme of studies carried out in the group. Proteins containing Fe/S centers are key players in bacterial stress response as Fe/S centers are both targets and generators of oxidative stress. Deficiencies in building Fe/S centers cause severe defects in any organism, including pathological disorders in humans. Because Fe/S centers are so reactive, their biogenesis requires complex machineries to make and deliver them to their final destination, the apo-targets. Our studies aimed at understanding how E. coli manages to carry out Fe/S biogenesis under different growth conditions. Studying Salmonella virulence offers an other model to study microbiol stress adaptation. Our current investigations aim at understanding how Salmonella resists oxidative and nitrosative stresses when it grows into macrophages. Role of the type-three-secretion system in Salmonella multiplication is also addressed. Our next studies will be adding imaging technology to our genetic and molecular expertises.